Confocal & multi-photon microscopes
Overview of confocal microscopes available at the expertise centre and their unique features:
Nikon A1R HD confocal & multiphoton microscope
Instrument specifications
- 4 lasers (405 nm, 488 nm, 561 nm, 640 nm)
- Galvoscanner and resonantscanner
- Perfect Focus System
- 4 detection channels (2 GaASP and 2PMT detectors) with choice of different emission filters
- 32-channel spectral detector unit
- Incubator with temperature regulation at the microscope level and CO2 at the cell culture level
- Multi-photon laser (Mai Tai TI:SAPPHIRE laser, 690-1040 nm)
- Non-descanned detectors
- Objective lenses:
- 60X water objective with automated correction ring (SR plan apo IR 60X WI, NA 1.3, WD 180µm)
- 60X oil (plan apo λ 60X oil, NA 1.4, WD 130 µm)
- 40X air (plan apo λ 40X, NA 0.9, WD 250 µm)
- 20X air (plan apo VC 20X, NA 0.8, WD 1000 µm)
Contact us for more information
Location
Faculty of Pharmaceutical Sciences,
Department of Pharmaceutics,
Ottergemsesteenweg 460,
B-9000, Ghent
Price
Price category 4 (Multiphoton)
Nikon A1R confocal microscope
Instrument specifications
- 4 lasers with in total 7 laser lines (405 nm, 457 nm, 476 nm, 488 nm, 514 nm, 561 nm, 639 nm)
- Galvoscanner and resonantscanner. Simultaneous use possible for photo-stimulation experiments (FRAP, FRET, ...)
- Piezo stage for fast and precise scanning in the z direction
- Perfect Focus System
- Simultaneous 4-channel PMT detection with choice of different emission filters
- 32-channel spectral PMT detector, useful for spectral imaging
- 2 separated lasers for Controlled Light Exposure Microscopy
- Incubator with temperature regulation at the microscope level and CO2 at the cell culture level
Contact us for more information
Location
Faculty Bioscience Engineering,
Vakgroep Biotechnologie, BW25
Campus Proeftuin, Gebouw N1, Lokaal 36.01.100.030
B-9000, Ghent
Price
Nikon C2 confocal microscope
Instrument specifications
- 4 Laser lines (405 nm, 488 nm, 560 nm, 637 nm)
- 3 PMT detectors and spectral detector, useful for spectral unmixing
- Photo-stimulation
- Fluorescence correlation spectroscopy (FCS) module
Contact us for more information
Location
Faculty of Pharmaceutical Sciences,
Department of Pharmaceutics,
Ottergemsesteenweg 460,
B-9000, Ghent
Price
Leica TCS-SP8 confocal microscope
Instrument specifications
- White light laser (470 – 670 nm), up to 8 freely tunable laser lines and 2 UV Laser lines (355 nm, 405 nm)
- 2 HyD (sensitive detection) and 2 PMT detectors
- Flexible tuning of emission bands via acousto-optical beam splitter (AOBS)
- Possibility of spectral imaging, (UV) photo-stimulation and resonant scanner
- Live cell imaging: Temperature and CO2 incubator
Contact us for more information
Location
Faculty of Medicine and Health Sciences,
Department of Basic Medical Sciences,
C. Heymanslaan 10, Blok B,
B-9000, Ghent
Price
Leica Stellaris 8 Falcon FLIM microscope
Instrument specifications
- FALCON: FLIM image acquisition, processing and analysis
- Objectives:
- HC PL APO 10x/0.40 CS2
- HC PL APO 40x/1.25 GLYC CORR CS2
- HC PL APO 63x/1.40 OIL CS2
- Excitation:
- White light laser (440 – 790 nm), up to 8 freely tunable laser lines
- 405 pulsed + CW laser
- Pulse picker (Pulse frequency 78, 39, 20, 10, 5, 2.5MHz)
- Detectors:
- pos 2: HyD X > 46% @ 500 nm, 410-750 nm, digital and counting
- pos 3: HyD S > 58% @ 500 nm, 410 – 850 nm, analog and counting
- pos 4: HyD X > 46% @ 500 nm, 410-750 nm, digital and counting
- pos 5: HyD R> 26% @ 635 nm, 410-830 nm, digital and counting (enhanced detection efficiency in NIR)
- Spectral Detection: 410 – 850 nm, fully tunable emission bands
- Galvano and 8kHz Resonant scanner
- Live cell imaging: Temperature and CO2 incubator
- sample holders:
- universal insert (standard Ibidi or glass slides, 35 mm dishes (glass bottom)
- insert for 96 well plate
- DMi8 CS body with:
- auto focus correction (AFC)
- automated scanning stage
- filtercubes for epifluorescence observation (DAPI, FITC, RHOD)
- LAS X STELLARIS Control Software with 3D visualisation and navigator
- Lightning (for deconvolution) an DSE Dynamic Signal Enhancement (rolling averaging for fast acquisition)
Contact us for more information
Location
Faculty of Medicine and Health Sciences,
Department of Human structure and repair,
Tissue Engineering and Biomaterials Group
C. Heymanslaan 10, Blok 6B3, entrance 46
B-9000, Ghent
Price
The pricing is subject to the grant used to acquire the instrument. Contact us for more information.